Natalie Tang
An Alternative, Native, and Covalent Approach to Halo-ChIP for Halo-Tagged Transcription Factors
Transcription factors (TFs) are proteins that bind to specific DNA sequences to regulate gene expression, impacting cell function, differentiation, and development. Isolation of TFs is a common practice in many research studies, and my goal this summer is to optimize an alternative approach that combines Halo-Tag and ChIP, two preexisting isolation protocols, for greater efficiency. By combining elements of both systems and engineering a new protein that forms covalent bonds with minimal loss of transcription factors, I aim to isolate TFs with enhanced accuracy for further research.
Message To Sponsor
I want to express my sincere gratitude for your support. Your generosity allows me to pursue my work with a new sense of confidence and enthusiasm. This opportunity is a significant step toward my academic and career goals, and I am eager to make the most of it. Thank you for making this journey possible!
Major: Bioengineering
Mentor: Robert Tjian and Claudia Cattoglio, Molecular & Cell Biology
Sponsor: Chandra Research Fellow - Chandra Fund