Studies of PRC2
I have been working in the Nogales lab since Summer 2016 through this academic year through URAP, working with a postdoctoral fellow (Vignesh Kasinath) and another undergraduate (Ashlee Feng) in the Nogales lab. My work in the Nogales lab is focused on understanding the structure and function of Polycomb Repressive Complex 2 (PRC2). So far we have been successful not just in the biochemical characterization which involves molecular cloning and protein purification, but also in preliminary electron microscopy studies (Negative stain and Cryo-EM). We have found conclusive evidence for the presence of PRC2 dimers which had previously only been postulated. Currently we are investigating the functional significance of these dimers and also studying interactions of PRC2 with RNA.