Effect of APOE2/3 on Neurodegenerative Health and Cholesterol Homeostasis in C. Elegans
APOE4 is a known risk factor for Alzheimer’s disease. In C. elegans, this is known to cause membranal breakdown due to negatively impacting cholesterol homeostasis. This causes issues in egg development in C. elegans, causing over-storage within the worms. At the Ma Lab, I will be further researching APOE2/3 as possible risk factors for neurodegenerative health in C. elegans and seeing whether they represent protective or harmful roles. Applications of techniques include microinjection, RNAi protocols, electroporation, and survival/behavioral assays.