Natalie Tang
An Alternative, Native, and Covalent Approach to Halo-ChIP for Halo-Tagged Transcription Factors
Transcription factors (TFs) are proteins that bind to specific DNA sequences to regulate gene expression, impacting cell function, differentiation, and development. Isolation of TFs is a common practice in many research studies, and my goal this summer is to optimize an alternative approach that combines Halo-Tag and ChIP, two preexisting isolation protocols, for greater efficiency. By combining elements of both systems and engineering a new protein that forms covalent bonds with minimal loss of transcription factors, I aim to isolate TFs with enhanced accuracy for further research.
Message To Sponsor
Thank you so much for affording me this opportunity to continue my research in the summer. This opportunity allowed me to further my skills and allow me to learn more without the pressure of classes during the school year. I appreciate your help and am grateful to have been awarded this chance to improve myself academically.
Major: Bioengineering
Mentor: Robert Tjian and Claudia Cattoglio, Molecular & Cell Biology
Sponsor: Chandra Research Fellow - Chandra Fund