Lillie Bui L&S Sciences
Reactive Oxygen in the Locus Coeruleus
Sleep is a fundamental aspect of human health. One way that our body regulates sleep is through sleep pressure, the balance between sleep and wakefulness. The dysregulation of sleep pressure is a common occurrence in patients with Parkinson’s disease, manifesting in sleep disturbances such as insomnia or excessive daytime sleepiness. It is undetermined if sleep problems and neurodegeneration have common mechanisms, but one potential area of research is in reactive oxygen species (ROS), which have been implicated in Parkinson’s disease. Emerging evidence indicates that ROS accumulate during steady activity of norepinephrine-releasing neurons in the locus coeruleus. These neurons are crucial for arousal and being in a state of wakefulness, and they are among the first to degenerate in Parkinson’s disease. In this project, I will use immunohistochemistry to investigate the specific type of ROS accumulation during prolonged locus coeruleus neuron activity, such as during sleep deprivation, and determine whether recovery sleep helps to promote ROS clearance. By furthering our understanding of ROS in locus coeruleus neurons, this project can help to improve how we approach sleep problems associated with Parkinson’s disease.