Jacqueline Dang Rose Hills
Optimizing Bacterial Binding to Nanosheets
I am working with my SURF mentor on optimizing binding of mannose conjugated nanosheets to E. Coli bacteria by controlling expression of pili. Loop are created on the nanosheet surface in an air-water interface by a rocking mechanism. These loops protrude from the nanosheet bilayer, mimicking glycoproteins and glycolipids that protrude from cell membrane surfaces.Type 1 pili, which are the structures that project from bacterial cell surfaces that govern binding, are primarily controlled by the fim operon. The fim operon has a multitude of constituent elements that code for fimbrae proteins (e.g. fimH, fimB, fimE, fimA). Our approach involves the removal of fim elements that naturally control the On/Off switch for pili expression. We would like to be able to have control over expression of Type 1 pili by exposure to varying amounts of the chemical inducer Isopropyl -D-1-thiogalactopyranoside (IPTG). The levels of binding and degrees of success will be observed via microscopy.