Arvin Javadi Rose Hills
Analyzing the Effects of Temperature on Protein Fragments of Human and Snake TRPA1 Ankyrin Repeats
TRPA1 ion channels are a specific example of a protein class that help the cell interact and respond to its environment. TRPA1 channels have been shown to consist generally of six trans-cell-membrane segments with two ends, an N-terminal and C-terminal end, with the N-terminal end extending into the cell. This endo-cellular region consists of 16 structural repeats called ankyrin repeat (AR) motifs. Studies have shown that TRPA1 channels in snake cells respond more to heat than chemical stimuli, in contrast to similar channels in mammals. Past research has also shown that by interchanging ARs 3-8 and 10-15 from humans to snakes (and viva versa), the relative responsiveness of TRPA1 to temperature changes. Our research is aimed at using circular dichroism (CD) to identify why these specific ARs changed the TRPA1’s response. We also hope to investigate how the order of the ankyrin repeats impacts motif response to temperature.