Aaron Kamajaya L&S Sciences
Essential amino acids for the regulated proteolysis of the master cell cycle regulator, CtrA
CtrA is a central regulatory protein controlling cell cycle progression in Caulobacter crescentus. The active phosphorylated form of CtrA directly controls the transcription of at least 95 cell-cycle-regulated genes as well as binding to sites near the origin to prevent initiation of chromosome replication. CtrA consists of receiver domain and DNA binding domain. CtrA activity is regulated by phosphorylation and degradation. My project is to elucidate the degradation mechanism of CtrA. We hypothesized that there are specific amino acid residues on CtrA receiver domain that are essential for its proteolysis. Thus, I will employ Genetics, Molecular Biology and Biochemical approaches to uncover the identity of these residues, and to determine how they mediate the effects of other factors which are known to be necessary for CtrA degradation.